› 6d3u

X-ray diffraction
2.21Å resolution

Complex structure of Ulvan lyase from Nonlaben Ulvanivorans- NLR48

Released:
DOI: 10.2210/pdb6d3u/pdb
PDB entry 6d3u coloured by chain, front view.
PDB entry 6d3u coloured by chain, side view.
PDB entry 6d3u coloured by chain, top view.
Source organism: Nonlabens ulvanivorans
Primary publication:
Structural and functional characterization of PL28 family ulvan lyase NLR48 from Nonlabens ulvanivorans.
Ulaganathan T, Banin E, Helbert W, Cygler M
J Biol Chem 293 11564-11573 (2018)
PMID: 29875159

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ulvan lyase NLR48 Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 33.46 KDa
Source organism: Nonlabens ulvanivorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A084JZF2 (Residues: 1-303; Coverage: 100%)
Gene names: IL45_01530, PLR_48
Sequence domains: PL28 ulvan lyases

Ligands and Environments

Carbohydrate polymer : NEW Components: 83Y, BDP, GCD
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand PO4 4 x PO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 82.464Å b: 102.664Å c: 103.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.
Bäumgen et al. (2021)
3 more

1 mention without citation

Structural and functional characterization of PL28 family ulvan lyase NLR48 from Nonlabens ulvanivorans.
Ulaganathan et al. (2018)