› 6de6

X-ray diffraction
2.1Å resolution

2.1 A resolution structure of histamine dehydrogenase from Rhizobium sp. 4-9

Released:
DOI: 10.2210/pdb6de6/pdb
PDB entry 6de6 coloured by chain, front view.
PDB entry 6de6 coloured by chain, side view.
PDB entry 6de6 coloured by chain, top view.
Source organism: Rhizobium sp. 4-9
Primary publication:
Structure of Rhizobium sp. 4-9 histamine dehydrogenase and analysis of the electron transfer pathway to an abiological electron acceptor.
Goyal P, Deay D, Seibold S, Candido ACL, Lovell S, Battaile KP, Wilson GS, Richter ML, Petillo PA
Arch Biochem Biophys 109612 (2023)
PMID: 37146865

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 696 amino acids
Theoretical weight: 77.1 KDa
Source organism: Rhizobium sp. 4-9
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60I59 (Residues: 1-693; Coverage: 100%)
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand FMN 2 x FMN
Ligand SF4 2 x SF4
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 103.223Å b: 105.314Å c: 140.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Modulating Substrate Specificity of Rhizobium sp. Histamine Dehydrogenase through Protein Engineering for Food Quality Applications.
Rodríguez-Núñez et al. (2023)