› 6du0

X-ray diffraction
1.82Å resolution

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y395L mutant

Released:
DOI: 10.2210/pdb6du0/pdb
PDB entry 6du0 coloured by chain, front view.
PDB entry 6du0 coloured by chain, side view.
PDB entry 6du0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Yeast require redox switching in DNA primase.
O'Brien E, Salay LE, Epum EA, Friedman KL, Chazin WJ, Barton JK
OpenAccess logo Proc Natl Acad Sci U S A 115 13186-13191 (2018)
PMID: 30541886

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase large subunit Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.31 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20457 (Residues: 316-512; Coverage: 37%)
Gene names: PRI2, YKL045W, YKL258
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand SF4 1 x SF4
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 38.462Å b: 50.83Å c: 90.175Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.273 0.324
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Redox Chemistry in the Genome: Emergence of the [4Fe4S] Cofactor in Repair and Replication.
Barton et al. (2019)
2 more

1 mention without citation

Yeast require redox switching in DNA primase.
O'Brien et al. (2018)