› 6dxu

X-ray diffraction
1.9Å resolution

Crystal Structure of Parabacteroides merdae Beta-Glucuronidase (GUS)

Released:
DOI: 10.2210/pdb6dxu/pdb
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PDB entry 6dxu coloured by chain, front view.
PDB entry 6dxu coloured by chain, side view.
PDB entry 6dxu coloured by chain, top view.
Source organism: Parabacteroides merdae ATCC 43184
Primary publication:
Active site flexibility revealed in crystal structures of Parabacteroides merdae β-glucuronidase from the human gut microbiome.
Little MS, Ervin SM, Walton WG, Tripathy A, Xu Y, Liu J, Redinbo MR
Protein Sci 27 2010-2022 (2018)
PMID: 30230652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241464 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 2, TIM barrel domain protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 830 amino acids
Theoretical weight: 94.87 KDa
Source organism: Parabacteroides merdae ATCC 43184
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

2 bound ligands:
Ligand BCN 3 x BCN
Ligand NA 8 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 91.371Å b: 163.393Å c: 120.607Å
α: 90° β: 103.351° γ: 90°
R-values:
R R work R free
0.154 0.154 0.184
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 review citations

A structural metagenomics pipeline for examining the gut microbiome.
Walker et al. (2022)
1 more

2 mentions without citation

A New Paradigm in the Relationship between Gut Microbiota and Breast Cancer: β-glucuronidase Enzyme Identified as Potential Therapeutic Target.
Fernández-Murga et al. (2023)
1 more

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