› 6e9o

X-ray diffraction
3.5Å resolution

E. coli D-galactonate:proton symporter mutant E133Q in the outward substrate-bound form

Released:
DOI: 10.2210/pdb6e9o/pdb
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PDB entry 6e9o coloured by chain, front view.
PDB entry 6e9o coloured by chain, side view.
PDB entry 6e9o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures suggest a mechanism for energy coupling by a family of organic anion transporters.
Leano JB, Batarni S, Eriksen J, Juge N, Pak JE, Kimura-Someya T, Robles-Colmenares Y, Moriyama Y, Stroud RM, Edwards RH
OpenAccess logo PLoS Biol 17 e3000260 (2019)
PMID: 31083648

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major facilitator superfamily (MFS) profile domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 460 amino acids
Theoretical weight: 50.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: J7QAK3 (Residues: 1-445; Coverage: 100%)
Gene names: A2J79_002380, A5U30_003247, B6R15_002606, BGM66_001314, C0P57_002075, C1Q91_001849, C2R31_003537, C719_001782, CF22_002085, CTR35_001134, EN85_004672, FOI11_017180, FOI11_06000, G3V95_06840, G4A38_07235, G4A47_06635, G5603_13780, HEP34_000485, HHH44_002014, HJQ60_003343, HVW04_15475, HVY77_00060, I6H02_13960, IH772_16335, J0541_001576, J5U05_002442, J8F57_000357, JNP96_00060, NY836_30400, P6223_003166, Q2V20_17465, QDW62_00060, QO046_16060, R8G00_15755, dgoT
Sequence domains: Major Facilitator Superfamily

Ligands and Environments

1 bound ligand:
Ligand J0M 2 x J0M
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 217.07Å b: 70.71Å c: 107.16Å
α: 90° β: 101.81° γ: 90°
R-values:
R R work R free
0.286 0.285 0.3
Expression system: Escherichia coli

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Citations

2 review citations

Molecular, Structural, Functional, and Pharmacological Sites for Vesicular Glutamate Transporter Regulation.
Pietrancosta et al. (2020)
1 more

9 mentions without citation

The mechanism and regulation of vesicular glutamate transport: Coordination with the synaptic vesicle cycle.
Eriksen et al. (2020)
8 more

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