› 6eic

X-ray diffraction
1.8Å resolution

Crystal structure of Rv0183, a Monoglyceride Lipase from Mycobacterium Tuberculosis

Released:
DOI: 10.2210/pdb6eic/pdb
PDB entry 6eic coloured by chain, front view.
PDB entry 6eic coloured by chain, side view.
PDB entry 6eic coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The crystal structure of monoacylglycerol lipase from M. tuberculosis reveals the basis for specific inhibition.
Aschauer P, Zimmermann R, Breinbauer R, Pavkov-Keller T, Oberer M
OpenAccess logo Sci Rep 8 8948 (2018)
PMID: 29895832

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoacylglycerol lipase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 279 amino acids
Theoretical weight: 30.3 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O07427 (Residues: 1-279; Coverage: 100%)
Gene name: Rv0183
Sequence domains: Serine aminopeptidase, S33

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand MPD 3 x MPD
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3112
Unit cell:
a: 85.825Å b: 85.825Å c: 196.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.251
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Emerging mechanisms by which endocannabinoids and their derivatives modulate bacterial populations within the gut microbiome.
Ellermann M. (2023)
1 more

7 mentions without citation

Identification of cell wall synthesis inhibitors active against Mycobacterium tuberculosis by competitive activity-based protein profiling.
Li et al. (2022)
6 more