› 6f7x

X-ray diffraction
2.42Å resolution

Crystal structure of dimethylated RSL - cucurbit[7]uril complex, F432 form

Released:
DOI: 10.2210/pdb6f7x/pdb
PDB entry 6f7x coloured by chain, front view.
PDB entry 6f7x coloured by chain, side view.
PDB entry 6f7x coloured by chain, top view.
Source organism: Ralstonia solanacearum
Primary publication:
Cucurbit[7]uril-Dimethyllysine Recognition in a Model Protein.
Guagnini F, Antonik PM, Rennie ML, O'Byrne P, Khan AR, Pinalli R, Dalcanale E, Crowley PB
Angew Chem Int Ed Engl 57 7126-7130 (2018)
PMID: 29673020

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative fucose-binding lectin protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 90 amino acids
Theoretical weight: 9.84 KDa
Source organism: Ralstonia solanacearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S4TLR1 (Residues: 2-91; Coverage: 99%)
Gene names: E7Z57_08365, HF909_06975, LBW55_09125, LBW59_25430, RUN39_v1_50103
Sequence domains: Fungal fucose-specific lectin
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand MFU 6 x MFU
Ligand QQ7 2 x QQ7
Ligand GOL 1 x GOL
1 modified residue:
Ligand MLY 9 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: F432
Unit cell:
a: 200.55Å b: 200.55Å c: 200.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Selective Recognition of Amino Acids and Peptides by Small Supramolecular Receptors.
Martins et al. (2020)
3 more

1 mention without citation

Segregated Protein-Cucurbit[7]uril Crystalline Architectures via Modulatory Peptide Tectons.
Ramberg et al. (2021)