› 6fh3

X-ray diffraction
1.85Å resolution

Protein arginine kinase McsB in the pArg-bound state

Released:
DOI: 10.2210/pdb6fh3/pdb
PDB entry 6fh3 coloured by chain, front view.
PDB entry 6fh3 coloured by chain, side view.
PDB entry 6fh3 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structure of McsB, a protein kinase for regulated arginine phosphorylation.
Suskiewicz MJ, Hajdusits B, Beveridge R, Heuck A, Vu LD, Kurzbauer R, Hauer K, Thoeny V, Rumpel K, Mechtler K, Meinhart A, Clausen T
Nat Chem Biol 15 510-518 (2019)
PMID: 30962626

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-arginine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 366 amino acids
Theoretical weight: 41.26 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DMM5 (Residues: 1-355; Coverage: 98%)
Gene name: mcsB
Sequence domains: ATP:guanido phosphotransferase, C-terminal catalytic domain

Ligands and Environments

2 bound ligands:
Ligand RPI 2 x RPI
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 49Å b: 77.11Å c: 94.13Å
α: 90° β: 98.08° γ: 90°
R-values:
R R work R free
0.182 0.181 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Applications of Bacterial Degrons and Degraders - Toward Targeted Protein Degradation in Bacteria.
Izert et al. (2021)
3 more

1 mention without citation

Structure of McsB, a protein kinase for regulated arginine phosphorylation.
Suskiewicz et al. (2019)