› 6fp8

X-ray diffraction
1.86Å resolution

mTFP1/DARPin 1238_E11 complex in space group C2

Released:
DOI: 10.2210/pdb6fp8/pdb
PDB entry 6fp8 coloured by chain, front view.
PDB entry 6fp8 coloured by chain, side view.
PDB entry 6fp8 coloured by chain, top view.
Source organisms:
Primary publication:
DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations.
Vigano MA, Bieli D, Schaefer JV, Jakob RP, Matsuda S, Maier T, Plückthun A, Affolter M
OpenAccess logo Biol Open 7 (2018)
PMID: 30237292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166730 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GFP-like fluorescent chromoprotein cFP484 Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.55 KDa
Source organism: Clavularia sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U6Y3 (Residues: 41-260; Coverage: 83%)
Sequence domains: Green fluorescent protein
DARPin1238_E11 Chain: B
Molecule details ›
Chain: B
Length: 182 amino acids
Theoretical weight: 19.88 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

3 bound ligands:
Ligand MPD 3 x MPD
Ligand SO4 4 x SO4
Ligand EDO 2 x EDO
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 135.953Å b: 75.012Å c: 48.295Å
α: 90° β: 90.73° γ: 90°
R-values:
R R work R free
0.15 0.148 0.176
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

1 review citation

Reflections on the use of protein binders to study protein function in developmental biology.
Aguilar et al. (2019)
2 other articles

2 mentions without citation

DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations.
Vigano et al. (2018)
1 more