6g1i

X-ray diffraction
0.99Å resolution

GH124 cellulase from Ruminiclostridium thermocellum in complex with Mn and fructosylated cellopentaose

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-107030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Dockerin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 25.35 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DCJ4 (Residues: 130-350; Coverage: 63%)
Gene name: Cthe_0435
Sequence domains: Cel124 C-terminal catalytic domain
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU, BGC
Carbohydrate polymer : NEW Components: BGC
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 71.14Å b: 74.173Å c: 77.328Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.131 0.147
Expression system: Escherichia coli