› 6g7l

X-ray diffraction
1.9Å resolution

Retinal isomerization in bacteriorhodopsin revealed by a femtosecond X-ray laser: 8.3 ms state structure

Released:
DOI: 10.2210/pdb6g7l/pdb
Model geometry
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PDB entry 6g7l coloured by chain, front view.
PDB entry 6g7l coloured by chain, side view.
PDB entry 6g7l coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser.
Nogly P, Weinert T, James D, Carbajo S, Ozerov D, Furrer A, Gashi D, Borin V, Skopintsev P, Jaeger K, Nass K, Båth P, Bosman R, Koglin J, Seaberg M, Lane T, Kekilli D, Brünle S, Tanaka T, Wu W, Milne C, White T, Barty A, Weierstall U, Panneels V, Nango E, Iwata S, Hunter M, Schapiro I, Schertler G, Neutze R, Standfuss J
Science 361 (2018)
PMID: 29903883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.27 KDa
Source organism: Halobacterium salinarum NRC-1
UniProt:
  • Canonical: P02945 (Residues: 1-262; Coverage: 100%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand LI1 7 x LI1
Ligand OLC 4 x OLC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: P63
Unit cell:
a: 62.32Å b: 62.32Å c: 111.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.272

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Citations

28 review citations

Membrane Protein-Lipid Interactions Probed Using Mass Spectrometry.
Bolla et al. (2019)
27 more

5 mentions without citation

An outlook on using serial femtosecond crystallography in drug discovery.
Mishin et al. (2019)
4 more

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