› 6gi9

X-ray diffraction
1.45Å resolution

Crystal structure of pentaerythritol tetranitrate reductase (PETNR) mutant I107L

Released:
DOI: 10.2210/pdb6gi9/pdb
PDB entry 6gi9 coloured by chain, front view.
PDB entry 6gi9 coloured by chain, side view.
PDB entry 6gi9 coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Nonequivalence of Second Sphere "Noncatalytic" Residues in Pentaerythritol Tetranitrate Reductase in Relation to Local Dynamics Linked to H-Transfer in Reactions with NADH and NADPH Coenzymes.
Iorgu AI, Baxter NJ, Cliff MJ, Levy C, Waltho JP, Hay S, Scrutton NS
OpenAccess logo ACS Catal 8 11589-11599 (2018)
PMID: 31119061

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 40.64 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: P71278 (Residues: 1-365; Coverage: 100%)
Gene name: onr
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand FMN 1 x FMN
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 57.32Å b: 70.32Å c: 88.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.126 0.166
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains.
Hedison et al. (2019)
4 other articles