› 6hdf

X-ray diffraction
1.4Å resolution

D170N variant of beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 1.4 A.

Released:
DOI: 10.2210/pdb6hdf/pdb
PDB entry 6hdf coloured by chain, front view.
PDB entry 6hdf coloured by chain, side view.
PDB entry 6hdf coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis Il1403
Entry authors: Wood HP, Robertson AJ, Bisson C, Waltho JP

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 24.24 KDa
Source organism: Lactococcus lactis subsp. lactis Il1403
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase

Ligands and Environments

2 bound ligands:
Ligand EDO 10 x EDO
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 38.77Å b: 119.31Å c: 53.17Å
α: 90° β: 94.8° γ: 90°
R-values:
R R work R free
0.174 0.172 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links