› 6hps

X-ray diffraction
3.1Å resolution

Near-infrared dual bioluminescence imaging in vivo using infra-luciferin

Released:
DOI: 10.2210/pdb6hps/pdb
PDB entry 6hps coloured by chain, front view.
PDB entry 6hps coloured by chain, side view.
PDB entry 6hps coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.
Stowe CL, Burley TA, Allan H, Vinci M, Kramer-Marek G, Ciobota DM, Parkinson GN, Southworth TL, Agliardi G, Hotblack A, Lythgoe MF, Branchini BR, Kalber TL, Anderson JC, Pule MA
OpenAccess logo Elife 8 (2019)
PMID: 31610848

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 543 amino acids
Theoretical weight: 59.98 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08659 (Residues: 4-546; Coverage: 99%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GKH 2 x GKH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 47.509Å b: 114.016Å c: 101.557Å
α: 90° β: 98.18° γ: 90°
R-values:
R R work R free
0.28 0.277 0.333
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.
Love et al. (2020)
7 more

1 mention without citation

Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.
Stowe et al. (2019)