› 6hsm

X-ray diffraction
2Å resolution

Structure of partially reduced RsrR in space group P2(1)2(1)2(1)

Released:
DOI: 10.2210/pdb6hsm/pdb
PDB entry 6hsm coloured by chain, front view.
PDB entry 6hsm coloured by chain, side view.
PDB entry 6hsm coloured by chain, top view.
Source organism: Streptomyces venezuelae ATCC 10712
Primary publication:
Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu, and His Residues.
Volbeda A, Martinez MTP, Crack JC, Amara P, Gigarel O, Munnoch JT, Hutchings MI, Darnault C, Le Brun NE, Fontecilla-Camps JC
J Am Chem Soc 141 2367-2375 (2019)
PMID: 30657661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rrf2 family transcriptional regulator Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 166 amino acids
Theoretical weight: 17.39 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2RGC9 (Residues: 1-160; Coverage: 100%)
Gene name: SVEN_6563
Sequence domains: Iron-dependent Transcriptional regulator

Ligands and Environments

6 bound ligands:
Ligand FES 8 x FES
Ligand MPD 28 x MPD
Ligand GOL 5 x GOL
Ligand CL 3 x CL
Ligand MG 1 x MG
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 66.65Å b: 129.84Å c: 170.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Modulation of MagR magnetic properties via iron-sulfur cluster binding.
Guo et al. (2021)
7 more