› 6ia9

X-ray diffraction
1.8Å resolution

urate oxidase under 2000 bar (220 MPa) of argon

Released:
DOI: 10.2210/pdb6ia9/pdb
PDB entry 6ia9 coloured by chain, front view.
PDB entry 6ia9 coloured by chain, side view.
PDB entry 6ia9 coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Comparative study of the effects of high hydrostatic pressure per se and high argon pressure on urate oxidase ligand stabilization
Prange T, Carpentier P, Dhaussy AC, Girard E, Colloc'h N
Acta Cryst. D 78 162-173 (2022)

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chains: aaa, aba
Molecule details ›
Chains: aaa, aba
Length: 295 amino acids
Theoretical weight: 33.5 KDa
Source organism: Aspergillus flavus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q00511 (Residues: 2-296; Coverage: 98%)
Gene names: Uox, uaZ
Sequence domains: Uricase

Ligands and Environments

4 bound ligands:
Ligand ACE 2 x ACE
Ligand NA 2 x NA
Ligand AZA 4 x AZA
Ligand AR 4 x AR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21212
Unit cell:
a: 78.552Å b: 96.682Å c: 105.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.243
Expression system: Komagataella pastoris

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