› 6imw

X-ray diffraction
2.1Å resolution

The complex structure of endo-beta-1,2-glucanase mutant (E262Q) from Talaromyces funiculosus with beta-1,2-glucan

Released:
DOI: 10.2210/pdb6imw/pdb
PDB entry 6imw coloured by chain, front view.
PDB entry 6imw coloured by chain, side view.
PDB entry 6imw coloured by chain, top view.
Source organism: Talaromyces funiculosus
Primary publication:
Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family.
Tanaka N, Nakajima M, Narukawa-Nara M, Matsunaga H, Kamisuki S, Aramasa H, Takahashi Y, Sugimoto N, Abe K, Terada T, Miyanaga A, Yamashita T, Sugawara F, Kamakura T, Komba S, Nakai H, Taguchi H
J Biol Chem 294 7942-7965 (2019)
PMID: 30926603

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->2)-glucosidic linkages in (1->2)-beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Endo-beta-1,2-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 56.6 KDa
Source organism: Talaromyces funiculosus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A4V8H013 (Residues: 1-449; Coverage: 100%)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 8 x NAG
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 92.169Å b: 117.924Å c: 93.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.236
Expression system: Komagataella pastoris

Quick links

Citations

4 citation in other articles

Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family.
Motouchi et al. (2023)
3 more

2 mentions without citation

Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family.
Tanaka et al. (2019)
1 more