› 6j2k

X-ray diffraction
1.44Å resolution

CTX-M-64 beta-lactamase S130T clavulanic acid complex

Released:
DOI: 10.2210/pdb6j2k/pdb
PDB entry 6j2k coloured by chain, front view.
PDB entry 6j2k coloured by chain, side view.
PDB entry 6j2k coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Insight into the Mechanism of Inhibitor Resistance in CTX-M-199, a CTX-M-64 Variant Carrying the S130T Substitution.
Cheng Q, Xu C, Chai J, Zhang R, Wai Chi Chan E, Chen S
ACS Infect Dis 6 577-587 (2020)
PMID: 31709791

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 30.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: C8CP57 (Residues: 29-291; Coverage: 100%)
Gene names: blaCTX-M-64, pHNAH46_020
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ISS 2 x ISS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 45.16Å b: 105.54Å c: 47.59Å
α: 90° β: 101.09° γ: 90°
R-values:
R R work R free
0.149 0.148 0.178
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

Molecular Mechanisms, Epidemiology, and Clinical Importance of β-Lactam Resistance in Enterobacteriaceae.
De Angelis et al. (2020)
2 more

1 mention without citation

Structural Comparisons of Cefotaximase (CTX-M-ase) Sub Family 1.
Shurina et al. (2021)