› 6jyc

X-ray diffraction
1.89Å resolution

Structure of dark-state marine bacterial chloride importer, NM-R3, with CW laser (ND-30%) at 95K.

Released:
DOI: 10.2210/pdb6jyc/pdb
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PDB entry 6jyc coloured by chain, front view.
PDB entry 6jyc coloured by chain, side view.
PDB entry 6jyc coloured by chain, top view.
Source organism: Nonlabens marinus S1-08
Primary publication:
Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family.
Yun JH, Ohki M, Park JH, Ishimoto N, Sato-Tomita A, Lee W, Jin Z, Tame JRH, Shibayama N, Park SY, Lee W
OpenAccess logo Sci Adv 6 eaay2042 (2020)
PMID: 32083178

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.91 KDa
Source organism: Nonlabens marinus S1-08
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand CL 2 x CL
Ligand OLA 11 x OLA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 102.539Å b: 49.213Å c: 69.186Å
α: 90° β: 110.06° γ: 90°
R-values:
R R work R free
0.17 0.168 0.21
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Biotechnological potentials of halophilic microorganisms and their impact on mankind.
Dutta et al. (2022)
1 more

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