› 6k63

X-ray diffraction
2.07Å resolution

The crystal structure of cytidine deaminase from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb6k63/pdb
PDB entry 6k63 coloured by chain, front view.
PDB entry 6k63 coloured by chain, side view.
PDB entry 6k63 coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Primary publication:
Biochemical and structural analysis of the Klebsiella pneumoniae cytidine deaminase CDA.
Liu W, Shang F, Chen Y, Lan J, Wang L, Chen J, Gao P, Ha NC, Quan C, Nam KH, Xu Y
Biochem Biophys Res Commun 519 280-286 (2019)
PMID: 31495495

Function and Biology Details

Reaction catalysed: 
2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytidine deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 294 amino acids
Theoretical weight: 31.51 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6TBN1 (Residues: 1-294; Coverage: 100%)
Gene names: KPN78578_25410, KPN_02584, cdd
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand DIO 12 x DIO
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3221
Unit cell:
a: 151.748Å b: 151.748Å c: 120.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia.
Chen et al. (2021)
1 more

2 mentions without citation

Deaminative chlorination of aminoheterocycles.
Ghiazza et al. (2022)
1 more