› 6kl0

X-ray diffraction
0.8Å resolution

Crystal structure of the S65T/F99S/M153T/V163A variant of perdeuterated GFP at pD 7.0

Released:
DOI: 10.2210/pdb6kl0/pdb
PDB entry 6kl0 coloured by chain, front view.
PDB entry 6kl0 coloured by chain, side view.
PDB entry 6kl0 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
X-ray crystallographic studies on the hydrogen isotope effects of green fluorescent protein at sub-ångström resolutions.
Tai Y, Takaba K, Hanazono Y, Dao HA, Miki K, Takeda K
Acta Crystallogr D Struct Biol 75 1096-1106 (2019)
PMID: 31793903

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.86 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-231; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand DOD 606 x DOD
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 50.826Å b: 62.402Å c: 68.875Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.107 0.106 0.12
Expression system: Escherichia coli BL21(DE3)

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