6kyl

X-ray diffraction
3.55Å resolution

Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 in Complex with (2R)-3-(phosphonooxy)propane-1,2-diyl dihexanoate

Released:
Primary publication:
Molecular mechanism of mitochondrial phosphatidate transfer by Ups1.
OpenAccess logo Commun Biol 3 468 (2020)
PMID: 32843686

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-129909 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial distribution and morphology protein 35 Chains: A, C
Molecule details ›
Chains: A, C
Length: 86 amino acids
Theoretical weight: 9.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: O60200 (Residues: 1-86; Coverage: 100%)
Gene names: MDM35, YKL053C-A
Sequence domains: Uncharacterised protein family (UPF0203)
Protein UPS1, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 189 amino acids
Theoretical weight: 21.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05776 (Residues: 1-175; Coverage: 100%)
Gene names: UPS1, YLR193C
Sequence domains: PRELI-like family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P41
Unit cell:
a: 90.14Å b: 90.14Å c: 81.319Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.289 0.287 0.31
Expression system: Escherichia coli