› 6lep

X-ray diffraction
2.6Å resolution

Crystal structure of thiosulfate transporter YeeE inactive mutant - C91A

Released:
DOI: 10.2210/pdb6lep/pdb
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PDB entry 6lep coloured by chain, front view.
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PDB entry 6lep coloured by chain, top view.
Source organism: Spirochaeta thermophila
Primary publication:
Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.
Tanaka Y, Yoshikaie K, Takeuchi A, Ichikawa M, Mori T, Uchino S, Sugano Y, Hakoshima T, Takagi H, Nonaka G, Tsukazaki T
OpenAccess logo Sci Adv 6 eaba7637 (2020)
PMID: 32923628

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate transporter TsuA Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 36.37 KDa
Source organism: Spirochaeta thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0GAP6 (Residues: 1-328; Coverage: 99%)
Gene names: Spith_0734, tsuA
Sequence domains: Sulphur transport

Ligands and Environments

2 bound ligands:
Ligand OLC 4 x OLC
Ligand THJ 1 x THJ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 73.91Å b: 94.61Å c: 101.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.235 0.286
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria.
Han et al. (2022)
1 more

1 mention without citation

Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake.
Tanaka et al. (2020)

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