› 6nlx

X-ray diffraction
1.8Å resolution

Crystal Structure of Glyceraldehyde-3-phosphate Dehydrogenase from Naegleria fowleri with bound NAD

Released:
DOI: 10.2210/pdb6nlx/pdb
PDB entry 6nlx coloured by chain, front view.
PDB entry 6nlx coloured by chain, side view.
PDB entry 6nlx coloured by chain, top view.
Source organism: Naegleria fowleri
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 341 amino acids
Theoretical weight: 37.67 KDa
Source organism: Naegleria fowleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4V8H039 (Residues: 1-333; Coverage: 100%)
Gene names: FDP41_008135, NF0055660
Sequence domains:
Structure domains: Dihydrodipicolinate Reductase; domain 2

Ligands and Environments


Cofactor: Ligand NAD 3 x NAD
1 bound ligand:
Ligand EDO 13 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 73.01Å b: 119.94Å c: 162.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.204
Expression system: Escherichia coli BL21(DE3)

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