› 6nrz

X-ray diffraction
2.1Å resolution

Crystal Structure of Lysyl-tRNA Synthetase from Chlamydia trachomatis complexed with L-lysine and Adenosine

Released:
DOI: 10.2210/pdb6nrz/pdb
PDB entry 6nrz coloured by chain, front view.
PDB entry 6nrz coloured by chain, side view.
PDB entry 6nrz coloured by chain, top view.
Source organism: Chlamydia trachomatis L2b/UCH-1/proctitis
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 534 amino acids
Theoretical weight: 61.18 KDa
Source organism: Chlamydia trachomatis L2b/UCH-1/proctitis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0BAN6 (Residues: 1-526; Coverage: 100%)
Gene names: CTLon_0150, lysS
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand LYS 2 x LYS
Ligand ADN 2 x ADN
Ligand EDO 28 x EDO
Ligand PG4 2 x PG4
Ligand NA 2 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 96.17Å b: 57.52Å c: 139.09Å
α: 90° β: 96.68° γ: 90°
R-values:
R R work R free
0.161 0.16 0.197
Expression system: Escherichia coli BL21(DE3)

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