› 6o08

X-ray diffraction
1.8Å resolution

Gluconobacter Ene-Reductase (GluER)

Released:
DOI: 10.2210/pdb6o08/pdb
PDB entry 6o08 coloured by chain, front view.
PDB entry 6o08 coloured by chain, side view.
PDB entry 6o08 coloured by chain, top view.
Source organism: Gluconobacter oxydans
Primary publication:
Photoexcitation of flavoenzymes enables a stereoselective radical cyclization.
Biegasiewicz KF, Cooper SJ, Gao X, Oblinsky DG, Kim JH, Garfinkle SE, Joyce LA, Sandoval BA, Scholes GD, Hyster TK
Science 364 1166-1169 (2019)
PMID: 31221855

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 40.36 KDa
Source organism: Gluconobacter oxydans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A1E8I9 (Residues: 1-361; Coverage: 100%)
Gene names: AD934_01855, AD950_09540, EDC20_102158, nox
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments

5 bound ligands:
Ligand FMN 1 x FMN
Ligand SO4 4 x SO4
Ligand ACT 5 x ACT
Ligand NA 1 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 44.695Å b: 49.469Å c: 156.223Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

25 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
24 more

1 mention without citation

Evidence of a Distinctive Enantioselective Binding Mode for the Photoinduced Radical Cyclization of α-Chloroamides in Ene-Reductases.
Capone et al. (2023)