› 6o0a

X-ray diffraction
1.7Å resolution

Crystal structure of flavohemoglobin from Malassezia yamatoensis with bound FAD and heme determined by iron SAD phasing

Released:
DOI: 10.2210/pdb6o0a/pdb
PDB entry 6o0a coloured by chain, front view.
PDB entry 6o0a coloured by chain, side view.
PDB entry 6o0a coloured by chain, top view.
Source organism: Malassezia yamatoensis
Primary publication:
HGT in the human and skin commensal Malassezia: A bacterially derived flavohemoglobin is required for NO resistance and host interaction.
Ianiri G, Coelho MA, Ruchti F, Sparber F, McMahon TJ, Fu C, Bolejack M, Donovan O, Smutney H, Myler P, Dietrich F, Fox D, LeibundGut-Landmann S, Heitman J
Proc Natl Acad Sci U S A 117 15884-15894 (2020)
PMID: 32576698

Function and Biology Details

Reaction catalysed: 
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nitric oxide dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.24 KDa
Source organism: Malassezia yamatoensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4V8H045 (Residues: 1-364; Coverage: 100%)
Sequence domains:
Structure domains: Globins

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 91.01Å b: 110.6Å c: 39.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Evolution of the human pathogenic lifestyle in fungi.
Rokas A. (2022)
6 more

3 mentions without citation

HGT in the human and skin commensal Malassezia: A bacterially derived flavohemoglobin is required for NO resistance and host interaction.
Ianiri et al. (2020)
2 more