› 6ofn

X-ray diffraction
1.65Å resolution

Crystal structure of green fluorescent protein (GFP); S65T, T203(3-OMeY); ih circular permutant (50-51)

Released:
DOI: 10.2210/pdb6ofn/pdb
PDB entry 6ofn coloured by chain, front view.
PDB entry 6ofn coloured by chain, side view.
PDB entry 6ofn coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins.
Lin CY, Romei MG, Oltrogge LM, Mathews II, Boxer SG
J Am Chem Soc 141 15250-15265 (2019)
PMID: 31450887

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 28.18 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 49-237; Coverage: 79%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:
Ligand CRO 2 x CRO
Ligand 3YM 2 x 3YM

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 48.19Å b: 67.763Å c: 58.849Å
α: 90° β: 101.51° γ: 90°
R-values:
R R work R free
0.164 0.162 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Frontiers in Multiscale Modeling of Photoreceptor Proteins.
Mroginski et al. (2021)
1 more

1 mention without citation

Unified Model for Photophysical and Electro-Optical Properties of Green Fluorescent Proteins.
Lin et al. (2019)