› 6okc

X-ray diffraction
1.55Å resolution

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and C12-crypto Acyl Carrier Protein, AcpP

Released:
DOI: 10.2210/pdb6okc/pdb
PDB entry 6okc coloured by chain, front view.
PDB entry 6okc coloured by chain, side view.
PDB entry 6okc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Gating mechanism of elongating β-ketoacyl-ACP synthases.
Mindrebo JT, Patel A, Kim WE, Davis TD, Chen A, Bartholow TG, La Clair JJ, McCammon JA, Noel JP, Burkart MD
OpenAccess logo Nat Commun 11 1727 (2020)
PMID: 32265440

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-oxoacyl-[acyl-carrier-protein] synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 42.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A953 (Residues: 1-406; Coverage: 100%)
Gene names: JW2320, b2323, fabB, fabC
Sequence domains:
Acyl carrier protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 78 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6A8 (Residues: 1-78; Coverage: 100%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

1 bound ligand:
Ligand MRJ 2 x MRJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 59.07Å b: 112.17Å c: 140.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.164
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Lessons in Organic Fluorescent Probe Discovery.
Wagh et al. (2021)
3 more

6 mentions without citation

Enzymology of standalone elongating ketosynthases.
Chen et al. (2022)
5 more