› 6okf

X-ray diffraction
2.5Å resolution

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and C16-crypto Acyl Carrier Protein, AcpP

Released:
DOI: 10.2210/pdb6okf/pdb
PDB entry 6okf coloured by chain, front view.
PDB entry 6okf coloured by chain, side view.
PDB entry 6okf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Gating mechanism of elongating β-ketoacyl-ACP synthases.
Mindrebo JT, Patel A, Kim WE, Davis TD, Chen A, Bartholow TG, La Clair JJ, McCammon JA, Noel JP, Burkart MD
OpenAccess logo Nat Commun 11 1727 (2020)
PMID: 32265440

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-oxoacyl-[acyl-carrier-protein] synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 406 amino acids
Theoretical weight: 42.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A953 (Residues: 1-406; Coverage: 100%)
Gene names: JW2320, b2323, fabB, fabC
Sequence domains:
Acyl carrier protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 78 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6A8 (Residues: 1-78; Coverage: 100%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand MRJ 2 x MRJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 58.939Å b: 100.781Å c: 79.26Å
α: 90° β: 108.64° γ: 90°
R-values:
R R work R free
0.179 0.176 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Lessons in Organic Fluorescent Probe Discovery.
Wagh et al. (2021)
5 more

1 mention without citation

Ketosynthase mutants enable short-chain fatty acid biosynthesis in E. coli.
Mains et al. (2023)