› 6ow4

X-ray diffraction
1.99Å resolution

Structure of the NADH-bound form of 20beta-Hydroxysteroid Dehydrogenase from Bifidobacterium adolescentis strain L2-32

Released:
DOI: 10.2210/pdb6ow4/pdb
PDB entry 6ow4 coloured by chain, front view.
PDB entry 6ow4 coloured by chain, side view.
PDB entry 6ow4 coloured by chain, top view.
Source organism: Bifidobacterium adolescentis L2-32
Primary publication:
Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32.
Doden HL, Pollet RM, Mythen SM, Wawrzak Z, Devendran S, Cann I, Koropatkin NM, Ridlon JM
J Biol Chem 294 12040-12053 (2019)
PMID: 31209107

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase, short chain dehydrogenase/reductase family protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 294 amino acids
Theoretical weight: 31.71 KDa
Source organism: Bifidobacterium adolescentis L2-32
Expression system: Escherichia coli
UniProt:
  • Canonical: A7A7R9 (Residues: 1-294; Coverage: 100%)
Gene name: BIFADO_01909
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

1 bound ligand:
Ligand NAD 8 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 165.078Å b: 134.71Å c: 96.396Å
α: 90° β: 100.15° γ: 90°
R-values:
R R work R free
0.19 0.187 0.238
Expression system: Escherichia coli

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Citations

3 review citations

Endobolome, a New Concept for Determining the Influence of Microbiota Disrupting Chemicals (MDC) in Relation to Specific Endocrine Pathogenesis.
Aguilera et al. (2020)
2 more

2 mentions without citation

Gut feelings about bacterial steroid-17,20-desmolase.
Ly et al. (2021)
1 more