› 6pbn

X-ray diffraction
1.65Å resolution

Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour

Released:
DOI: 10.2210/pdb6pbn/pdb
PDB entry 6pbn coloured by chain, front view.
PDB entry 6pbn coloured by chain, side view.
PDB entry 6pbn coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Staphylopine and pseudopaline dehydrogenase from bacterial pathogens catalyze reversible reactions and produce stereospecific metallophores.
McFarlane JS, Zhang J, Wang S, Lei X, Moran GR, Lamb AL
J Biol Chem 294 17988-18001 (2019)
PMID: 31615895

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudopaline synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 49.58 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUX5 (Residues: 1-433; Coverage: 100%)
Gene names: PA4835, cntM, zrmC
Sequence domains: Staphylopine dehydrogenase

Ligands and Environments

4 bound ligands:
Ligand NAP 2 x NAP
Ligand EDO 5 x EDO
Ligand AKG 1 x AKG
Ligand O77 1 x O77
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 181.023Å b: 53.842Å c: 96.867Å
α: 90° β: 98.77° γ: 90°
R-values:
R R work R free
0.174 0.174 0.21
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Systematic identification of molecular mediators of interspecies sensing in a community of two frequently coinfecting bacterial pathogens.
Zarrella et al. (2022)

1 mention without citation

Staphylopine and pseudopaline dehydrogenase from bacterial pathogens catalyze reversible reactions and produce stereospecific metallophores.
McFarlane et al. (2019)