› 6pis

X-ray diffraction
2.77Å resolution

Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Released:
DOI: 10.2210/pdb6pis/pdb
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PDB entry 6pis coloured by chain, front view.
PDB entry 6pis coloured by chain, side view.
PDB entry 6pis coloured by chain, top view.
Source organisms:
Primary publication:
The mechanosensitive ion channel TRAAK is localized to the mammalian node of Ranvier.
Brohawn SG, Wang W, Handler A, Campbell EB, Schwarz JR, MacKinnon R
OpenAccess logo Elife 8 (2019)
PMID: 31674909

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231675 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Potassium channel subfamily K member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 33.73 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O88454 (Residues: 1-275; Coverage: 69%)
Gene names: Kcnk4, Traak
Sequence domains:
ANTIBODY FAB FRAGMENT LIGHT CHAIN Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.53 KDa
Source organism: Nothocricetulus migratorius
ANTIBODY FAB FRAGMENT HEAVY CHAIN Chains: H, I

Ligands and Environments

1 bound ligand:
Ligand K 6 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 72.45Å b: 154.51Å c: 202.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.286
Expression system: Komagataella pastoris

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Citations

15 review citations

Discoveries in structure and physiology of mechanically activated ion channels.
Kefauver et al. (2020)
14 more

1 mention without citation

The mechanosensitive ion channel TRAAK is localized to the mammalian node of Ranvier.
Brohawn et al. (2019)

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