› 6pth

X-ray diffraction
3.05Å resolution

Crystal structure of a DnaN sliding clamp (DNA polymerase III subunit beta) from Pseudomonas aeruginosa bound to griselimycin

Released:
DOI: 10.2210/pdb6pth/pdb
PDB entry 6pth coloured by chain, front view.
PDB entry 6pth coloured by chain, side view.
PDB entry 6pth coloured by chain, top view.
Source organisms:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 51.84 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I7C4 (Residues: 1-367; Coverage: 100%)
Gene names: PA0002, dnaN
Sequence domains:
Griselimycin Chain: G
Molecule details ›
Chain: G
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
3 modified residues:
Ligand MVA 2 x MVA
Ligand MP8 2 x MP8
Ligand NZC 1 x NZC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6422
Unit cell:
a: 139.65Å b: 139.65Å c: 173.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.224
Expression systems:
  • Escherichia coli
  • Not provided

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