› 6q2m

X-ray diffraction
2.75Å resolution

Crystal structure of Photinus pyralis Luciferase Pps6 mutant in complex with DLSA

Released:
DOI: 10.2210/pdb6q2m/pdb
PDB entry 6q2m coloured by chain, front view.
PDB entry 6q2m coloured by chain, side view.
PDB entry 6q2m coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Mutagenesis and Structural Studies Reveal the Basis for the Activity and Stability Properties That Distinguish the Photinus Luciferases scintillans and pyralis.
Branchini BR, Fontaine DM, Southworth TL, Huta BP, Racela A, Patel KD, Gulick AM
Biochemistry 58 4293-4303 (2019)
PMID: 31560532

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 555 amino acids
Theoretical weight: 61.23 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 6 x SO4
Ligand DYD 4 x DYD
Ligand EDO 39 x EDO
Ligand SLU 3 x SLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 66.268Å b: 95.455Å c: 152.02Å
α: 90° β: 101.556° γ: 90°
R-values:
R R work R free
0.195 0.194 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The elusive relationship between structure and colour emission in beetle luciferases.
Carrasco-López et al. (2021)
6 other articles

1 mention without citation

Mutagenesis and Structural Studies Reveal the Basis for the Activity and Stability Properties That Distinguish the Photinus Luciferases scintillans and pyralis.
Branchini et al. (2019)