› 6qfo

X-ray diffraction
2.3Å resolution

EngBF DARPin Fusion 9b 3G124

Released:
DOI: 10.2210/pdb6qfo/pdb
PDB entry 6qfo coloured by chain, front view.
PDB entry 6qfo coloured by chain, side view.
PDB entry 6qfo coloured by chain, top view.
Source organism: Bifidobacterium longum
Primary publication:
Structural analysis of biological targets by host:guest crystal lattice engineering.
Ernst P, Plückthun A, Mittl PRE
OpenAccess logo Sci Rep 9 15199 (2019)
PMID: 31645583

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PEGA domain-containing protein,PEGA domain-containing protein,EngBF DARPin fusion 9b 3G124 Chain: A
Molecule details ›
Chain: A
Length: 1362 amino acids
Theoretical weight: 148.32 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand MES 1 x MES
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P65
Unit cell:
a: 193.47Å b: 193.47Å c: 123.77Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.204
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Folding and Stability of Ankyrin Repeats Control Biological Protein Function.
Kumar et al. (2021)
4 other articles

1 mention without citation

Structural analysis of biological targets by host:guest crystal lattice engineering.
Ernst et al. (2019)