› 6qhe

X-ray diffraction
1.83Å resolution

Alcohol Dehydrogenase from Arthrobacter sp. TS-15 in complex with NAD+

Released:
DOI: 10.2210/pdb6qhe/pdb
PDB entry 6qhe coloured by chain, front view.
PDB entry 6qhe coloured by chain, side view.
PDB entry 6qhe coloured by chain, top view.
Source organism: Arthrobacter
Primary publication:
Two Enantiocomplementary Ephedrine Dehydrogenases from Arthrobacter sp. TS-15 with Broad Substrate Specificity
Shanati T, Lockie C, Beloti L, Grogan G, Ansorge-Schumacher M
ACS Catalysis 9 6202-6211 (2019)

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoephedrine dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 275 amino acids
Theoretical weight: 28.7 KDa
Source organism: Arthrobacter
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A545BBS8 (Residues: 1-274; Coverage: 100%)
Gene name: EU811_20440
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments


Cofactor: Ligand NAD 5 x NAD
1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 80.731Å b: 119.041Å c: 348.578Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links