› 6s0g

X-ray diffraction
1.45Å resolution

Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria

Released:
DOI: 10.2210/pdb6s0g/pdb
PDB entry 6s0g coloured by chain, front view.
PDB entry 6s0g coloured by chain, side view.
PDB entry 6s0g coloured by chain, top view.
Source organism: Galdieria sulphuraria
Primary publication:
Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.
Robescu MS, Niero M, Hall M, Cendron L, Bergantino E
Appl Microbiol Biotechnol 104 2051-2066 (2020)
PMID: 31930452

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 401 amino acids
Theoretical weight: 45.49 KDa
Source organism: Galdieria sulphuraria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M2XAQ9 (Residues: 1-381; Coverage: 100%)
Gene name: Gasu_54250
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand FMN 1 x FMN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Unit cell:
a: 56.745Å b: 76.662Å c: 88.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Old yellow enzymes: structures and structure-guided engineering for stereocomplementary bioreduction.
Shi et al. (2020)
4 other articles

1 mention without citation

Structural and functional characterization of a new thermophilic-like OYE from Aspergillus flavus.
Li et al. (2024)