6s4d

X-ray diffraction
1.8Å resolution

Crystal Structure of the Homospermidine Synthase (HSS) variant W229F from Blastochloris viridis in Complex with NAD

Released:

Function and Biology Details

Reaction catalysed:
2 putrescine = sym-homospermidine + NH(3)
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-128445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homospermidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 53 KDa
Source organism: Blastochloris viridis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32323 (Residues: 2-477; Coverage: 100%)
Gene name: hss
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 60.11Å b: 110.009Å c: 158.163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.208
Expression system: Escherichia coli