› 6s65

X-ray diffraction
1.75Å resolution

Crystal Structure of the Homospermidine Synthase (HSS) variant N135F from Blastochloris viridis in Complex with NAD

Released:
DOI: 10.2210/pdb6s65/pdb
PDB entry 6s65 coloured by chain, front view.
PDB entry 6s65 coloured by chain, side view.
PDB entry 6s65 coloured by chain, top view.
Source organism: Blastochloris viridis
Entry authors: Helfrich F, Scheidig AJ

Function and Biology Details

Reaction catalysed: 
2 putrescine = sym-homospermidine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homospermidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 53.07 KDa
Source organism: Blastochloris viridis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32323 (Residues: 2-477; Coverage: 100%)
Gene name: hss
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 60.703Å b: 110.401Å c: 159.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli

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