› 6sea

X-ray diffraction
1.87Å resolution

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with lactose bound in deep mode

Released:
DOI: 10.2210/pdb6sea/pdb
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Source organism: Arthrobacter sp. 32cB
Primary publication:
Active Site Architecture and Reaction Mechanism Determination of Cold Adapted β-d-galactosidase from Arthrobacter sp. 32cB.
Rutkiewicz M, Bujacz A, Wanarska M, Wierzbicka-Wos A, Cieslinski H
OpenAccess logo Int J Mol Sci 20 (2019)
PMID: 31484304

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 1010 amino acids
Theoretical weight: 109.76 KDa
Source organism: Arthrobacter sp. 32cB
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A023UGN9 (Residues: 1-1010; Coverage: 100%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, BGC
Carbohydrate polymer
3 bound ligands:
Ligand NA 4 x NA
Ligand ACT 5 x ACT
Ligand GAL 2 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 138.613Å b: 138.613Å c: 127.347Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.205
Expression system: Escherichia coli

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Citations

2 review citations

Some Clues about Enzymes from Psychrophilic Microorganisms.
Rapuano et al. (2022)
1 more

2 mentions without citation

Active Site Architecture and Reaction Mechanism Determination of Cold Adapted β-d-galactosidase from Arthrobacter sp. 32cB.
Rutkiewicz et al. (2019)
1 more

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