› 6tsb

X-ray diffraction
2.1Å resolution

Crystal structure of the Chitinase Domain of the Spore Coat Protein CotE from Clostridium difficile

Released:
DOI: 10.2210/pdb6tsb/pdb
PDB entry 6tsb coloured by chain, front view.
PDB entry 6tsb coloured by chain, side view.
PDB entry 6tsb coloured by chain, top view.
Source organism: Clostridioides difficile 630
Primary publication:
Crystal structures of the GH18 domain of the bifunctional peroxiredoxin-chitinase CotE from Clostridium difficile.
Whittingham JL, Hanai S, Brannigan JA, Ferreira WT, Dodson EJ, Turkenburg JP, Cartwright J, Cutting SM, Wilkinson AJ
OpenAccess logo Acta Crystallogr F Struct Biol Commun 76 241-249 (2020)
PMID: 32510464

Function and Biology Details

Reactions catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin Chain: AAA
Molecule details ›
Chain: AAA
Length: 367 amino acids
Theoretical weight: 40.77 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q18BV5 (Residues: 348-712; Coverage: 51%)
Gene names: CD630_14330, cotE
Sequence domains: Glycosyl hydrolases family 18

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6122
Unit cell:
a: 82.076Å b: 82.076Å c: 325.883Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Clostridioides difficile spore: coat assembly and formation.
Zeng et al. (2022)

1 mention without citation

Crystal structures of the GH18 domain of the bifunctional peroxiredoxin-chitinase CotE from Clostridium difficile.
Whittingham et al. (2020)