› 6u1v

X-ray diffraction
1.75Å resolution

Crystal structure of acyl-ACP/acyl-CoA dehydrogenase from allylmalonyl-CoA and FK506 biosynthesis, TcsD

Released:
DOI: 10.2210/pdb6u1v/pdb
PDB entry 6u1v coloured by chain, front view.
PDB entry 6u1v coloured by chain, side view.
PDB entry 6u1v coloured by chain, top view.
Source organism: Streptomyces tsukubensis NRRL18488
Primary publication:
Structural Mechanism of Regioselectivity in an Unusual Bacterial Acyl-CoA Dehydrogenase.
Blake-Hedges JM, Pereira JH, Cruz-Morales P, Thompson MG, Barajas JF, Chen J, Krishna RN, Chan LJG, Nimlos D, Alonso-Martinez C, Baidoo EEK, Chen Y, Gin JW, Katz L, Petzold CJ, Adams PD, Keasling JD
J Am Chem Soc 142 835-846 (2020)
PMID: 31793780

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-[acyl-carrier-protein] dehydrogenase MbtN Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 42.32 KDa
Source organism: Streptomyces tsukubensis NRRL18488
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I2MTW3 (Residues: 1-386; Coverage: 100%)
Gene name: STSU_032215
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FDA 4 x FDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 69.52Å b: 100.06Å c: 148.51Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.148 0.146 0.179
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Functional Studies and Revision of the NFAT-133/TM-123 Biosynthetic Pathway in Streptomyces pactum.
Zhou et al. (2022)
2 more