› 6vil

X-ray diffraction
3.3Å resolution

Crystal structure of mouse BAHCC1 BAH domain in complex with H3K27me3

Released:
DOI: 10.2210/pdb6vil/pdb
PDB entry 6vil coloured by chain, front view.
PDB entry 6vil coloured by chain, side view.
PDB entry 6vil coloured by chain, top view.
Source organisms:
Primary publication:
BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis.
Fan H, Lu J, Guo Y, Li D, Zhang ZM, Tsai YH, Pi WC, Ahn JH, Gong W, Xiang Y, Allison DF, Geng H, He S, Diao Y, Chen WY, Strahl BD, Cai L, Song J, Wang GG
Nat Genet 52 1384-1396 (2020)
PMID: 33139953

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159410 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BAH and coiled-coil domain-containing protein 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 160 amino acids
Theoretical weight: 18.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3UHR0 (Residues: 2485-2643; Coverage: 6%)
Gene names: Bahcc1, Kiaa1447
Sequence domains: BAH domain
Histone H3.1 Chains: I, J, L, M
Molecule details ›
Chains: I, J, L, M
Length: 14 amino acids
Theoretical weight: 1.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 22-34; Coverage: 10%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 4 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P31
Unit cell:
a: 184.176Å b: 184.176Å c: 70.276Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.248 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

The language of chromatin modification in human cancers.
Zhao et al. (2021)
7 more