› 6w7o

X-ray diffraction
2.17Å resolution

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170300 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase BTK Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 32.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: Q06187 (Residues: 384-659; Coverage: 42%)
Gene names: AGMX1, ATK, BPK, BTK
Sequence domains: Protein tyrosine and serine/threonine kinase
Baculoviral IAP repeat-containing protein 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 99 amino acids
Theoretical weight: 11.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13490 (Residues: 260-352; Coverage: 15%)
Gene names: API1, BIRC2, MIHB, RNF48
Sequence domains: Inhibitor of Apoptosis domain

Ligands and Environments

2 bound ligands:
Ligand TL7 2 x TL7
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 37.98Å b: 56.33Å c: 98.12Å
α: 104.98° β: 100.89° γ: 90.13°
R-values:
R R work R free
0.198 0.196 0.24
Expression systems:
  • Spodoptera aff. frugiperda 2 RZ-2014
  • Escherichia coli

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