› 6wuk

X-ray diffraction
1.6Å resolution

Crystal structure of mouse DXO in complex with CoA

Released:
DOI: 10.2210/pdb6wuk/pdb
PDB entry 6wuk coloured by chain, front view.
PDB entry 6wuk coloured by chain, side view.
PDB entry 6wuk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
DXO/Rai1 enzymes remove 5'-end FAD and dephospho-CoA caps on RNAs.
Doamekpor SK, Grudzien-Nogalska E, Mlynarska-Cieslak A, Kowalska J, Kiledjian M, Tong L
OpenAccess logo Nucleic Acids Res 48 6136-6148 (2020)
PMID: 32374864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decapping and exoribonuclease protein Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 47.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70348 (Residues: 1-397; Coverage: 100%)
Gene names: Dom3z, Dxo, Ng6
Sequence domains: RAI1 like PD-(D/E)XK nuclease

Ligands and Environments

1 bound ligand:
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 49.862Å b: 87.665Å c: 54.023Å
α: 90° β: 112.24° γ: 90°
R-values:
R R work R free
0.186 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Re-evaluation of Diadenosine Tetraphosphate (Ap4A) From a Stress Metabolite to Bona Fide Secondary Messenger.
Ferguson et al. (2020)
3 more