› 6wxa

X-ray diffraction
2.3Å resolution

Crystal structure of truncated Streptococcal bacteriophage hyaluronidase complexed with unsaturated hyaluronan hexa-saccharides

Released:
DOI: 10.2210/pdb6wxa/pdb
PDB entry 6wxa coloured by chain, front view.
PDB entry 6wxa coloured by chain, side view.
PDB entry 6wxa coloured by chain, top view.
Source organism: Streptococcus pyogenes phage H4489A
Entry authors: Deivanayagam C, Schormann N

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Hyaluronoglucosaminidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 293 amino acids
Theoretical weight: 31.53 KDa
Source organism: Streptococcus pyogenes phage H4489A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15316 (Residues: 87-371; Coverage: 77%)
Gene names: HYLP, HYLP1
Sequence domains: Hyaluronidase protein (HylP)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BDP, GCD
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BDP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BDP, GCD
Carbohydrate polymer
1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 67.296Å b: 86.492Å c: 168.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.243
Expression system: Escherichia coli BL21(DE3)

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