› 6y9j

X-ray diffraction
1.1Å resolution

Crystal Structure of subtype-switched Epithelial Adhesin 1 to 9 A domain (Epa1-CBL2Epa9) from Candida glabrata in complex with beta-lactose

Released:
DOI: 10.2210/pdb6y9j/pdb
PDB entry 6y9j coloured by chain, front view.
PDB entry 6y9j coloured by chain, side view.
PDB entry 6y9j coloured by chain, top view.
Source organism: [Candida] glabrata
Primary publication:
Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.
Hoffmann D, Diderrich R, Reithofer V, Friederichs S, Kock M, Essen LO, Mösch HU
J Biol Chem 295 12512-12524 (2020)
PMID: 32669365

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PA14 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.45 KDa
Source organism: [Candida] glabrata
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q6FUW5 (Residues: 31-271; Coverage: 24%)
Gene names: CAGL0E06644g, EPA1
Sequence domains: GLEYA domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 1 x CA
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 74.22Å b: 104.14Å c: 69.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.167
Expression system: Escherichia coli K-12

Quick links

Citations

5 citation in other articles

A novel class of Candida glabrata cell wall proteins with β-helix fold mediates adhesion in clinical isolates.
Reithofer et al. (2021)
4 more

2 mentions without citation

Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.
Hoffmann et al. (2020)
1 more