› 6yu2

X-ray diffraction
3.1Å resolution

Crystal structure of MhsT in complex with L-isoleucine

Released:
DOI: 10.2210/pdb6yu2/pdb
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PDB entry 6yu2 coloured by chain, front view.
PDB entry 6yu2 coloured by chain, side view.
PDB entry 6yu2 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
A non-helical region in transmembrane helix 6 of hydrophobic amino acid transporter MhsT mediates substrate recognition.
Focht D, Neumann C, Lyons J, Eguskiza Bilbao A, Blunck R, Malinauskaite L, Schwarz IO, Javitch JA, Quick M, Nissen P
EMBO J 40 e105164 (2021)
PMID: 33155685

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium-dependent transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 48.36 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Lactococcus lactis
UniProt:
  • Canonical: Q9KDT3 (Residues: 2-453; Coverage: 100%)
Gene name: BH1128
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand BOG 6 x BOG
Ligand NA 4 x NA
Ligand ILE 2 x ILE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 43.95Å b: 97.29Å c: 110.94Å
α: 90° β: 96.14° γ: 90°
R-values:
R R work R free
0.278 0.277 0.305
Expression system: Lactococcus lactis

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Citations

9 citation in other articles

Structural insights into the inhibition of glycine reuptake.
Shahsavar et al. (2021)
8 more

1 mention without citation

Lactococcus lactis, an Attractive Cell Factory for the Expression of Functional Membrane Proteins.
Frelet-Barrand A. (2022)

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