› 7fs9

X-ray diffraction
1.72Å resolution

Structure of liver pyruvate kinase in complex with allosteric modulator 22

Released:
DOI: 10.2210/pdb7fs9/pdb
PDB entry 7fs9 coloured by chain, front view.
PDB entry 7fs9 coloured by chain, side view.
PDB entry 7fs9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
Nain-Perez A, Nilsson O, Lulla A, Håversen L, Brear P, Liljenberg S, Hyvönen M, Borén J, Grøtli M
Eur J Med Chem 250 115177 (2023)
PMID: 36753880

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKLR Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 447 amino acids
Theoretical weight: 48.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30613 (Residues: 34-160, 262-574; Coverage: 77%)
  • Best match: P30613-2 (Residues: 3-129, 231-543)
Gene names: PK1, PKL, PKLR
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand FBP 8 x FBP
Ligand OXL 8 x OXL
Ligand MG 8 x MG
Ligand K 8 x K
Ligand OB0 4 x OB0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 208.83Å b: 112.994Å c: 189.086Å
α: 90° β: 91.18° γ: 90°
R-values:
R R work R free
0.194 0.193 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Effects of Salinity Stress on Histological Changes, Glucose Metabolism Index and Transcriptomic Profile in Freshwater Shrimp, Macrobrachium nipponense.
Li et al. (2023)

2 mentions without citation

A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.
Roldán-Salgado et al. (2022)
1 more